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cwlT [2018-06-18 14:57:10]
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cwlT [2018-06-18 14:57:10]

cell wall hydrolase, required for conjugation of ICEBs1, part of the type IV secretion system for DNA transfer , C-terminal domain hydrolyzes bond between D-Glu and m-DAP
locus
BSU04970
pI
8.63
mw
36.39 kDa
protein length
329 aa Sequence Blast
gene length
987 bp Sequence Blast
function
conjugative transfer of ICEBs1
product
cell wall hydrolase
synonyms
yddH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.2|Mobile genetic elements] → [category|SW 5.2.1|ICEBs1]

    • Gene

    Coordinates
    544,022 → 545,011

    The protein

    Catalyzed reaction/ biological activity

  • hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D-γ-glutamate and meso-diaminopimelic acid) [Pubmed|18305117]

    • Protein family

  • nlpC/p60 family (according to Swiss-Prot)

    • [SW|Domains]

  • N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine [Pubmed|18305117]
  • C-terminal endopeptidase domain cleaves the bond between D-γ-glutamate and meso-diaminopimelic acid [Pubmed|18305117]

    • Structure

  • [PDB|4FDY] (from Staphylococcus aureus, 42% identity) [pubmed|24051416]

    • [SW|Localization]

  • secreted (with signal peptide) [Pubmed|24532767]
  • may be a lipoprotein, but the lipid anchor is not required for function [Pubmed|24532767]

    • Expression and Regulation

    Operons

    genes
    [gene|EE2A4068CBD7A2F852E582E2EDA4148381F1CD61|xis]-[gene|2227923C603B290AB276238F789D71584FB07DF3|ydzL]-[gene|34451538F10B4ABCFC8A236C8C905A20D989EBCF|ydcO]-[gene|C042792D9A643DCFF4A851EFECA3CF2C31E1EDAF|ydcP]-[gene|4BCAA5939CB7F0DF23495BC92C9EF0A0149296A0|ydcQ]-[gene|8E179976C35FE09F92A191D00E497ED4BF28F571|nicK]-[gene|886B2132717BA4C72433ABE351C5791C9DE1432A|S172]-[gene|1699CFBF5400CAF43C6DAE8ADCCE5A360B31F2E9|ydcS]-[gene|9D4918852A1856B1CDD72D49AEA75E51A8602BB6|ydcT]-[gene|62CEABA069CA2970F22B7E0C369D540A2A027A8D|yddA]-[gene|A0AFD543582D2FE8B1139CF69B7B1F7FF0F80265|conB]-[gene|F273A8817E66B44D58FC2BCB6576A30D8616B750|conC]-[gene|F1F898D7A9FDF78D2E5E8F3BF605E0F735D0542A|conD]-[gene|D20F724D8C3E8C758A9FADF133CD43F066BD8D48|conE]-[gene|B3A07E2A61151C33B7C65D1BC58B97BFDB76852D|yddF]-[gene|3356A43B83E2D4D66098F17A751F0912C1553398|conG]-[gene|8D9D81E05BAA8697F002BB1B8DBC5ABBA557353F|cwlT]-[gene|2E4FF80C857B3F40BA835814EC40D6562721AEFE|yddI]-[gene|2FB1C9435AE924F8E450AFA81F800196A871F2D0|yddJ]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|DD1C8F3A4809785BD6A6047D39B42AB2C605E161|ImmR]: repression, [Pubmed|17511812], in [regulon|DD1C8F3A4809785BD6A6047D39B42AB2C605E161|ImmR regulon]

    • regulation

  • strongly induced in the presence of salt (1.2 M NaCl) [pubmed|32419322]
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    Biological materials

    Mutant

  • BKE04970 (Δ[gene|8D9D81E05BAA8697F002BB1B8DBC5ABBA557353F|cwlT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA
  • BKK04970 (Δ[gene|8D9D81E05BAA8697F002BB1B8DBC5ABBA557353F|cwlT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA

    • References

  • 18305117,24532767,24051416